Topic
Degradants
Page 10 of 13 — Deamidation at plus one dalton, oxidation at plus sixteen, truncation sequences, and what a warm transit actually does.
Messages — page 10 of 13
peak_splitplus sixteen, is that always oxidation#hplc-massspec · 2026-04-05baseline_drifta warm transit can put a visible degradant peak on a chromatogram. that is what 23 days at ambient does nobody runs enough…#hplc-massspec · 2026-04-09HPLC_Hankhow would a truncation sequence show up#hplc-massspec · 2026-04-09hydrate_hanaoxidation is about plus 15.99 Da and is usually methionine or tryptophan, from memory#hplc-massspec · 2026-04-09tarpit_tamplus sixteen, is that always oxidation#hplc-massspec · 2026-04-10swirl_not_shakeplus 16 is oxidation#hplc-massspec · 2026-04-10juniper_joinshow would a truncation sequence show up#hplc-massspec · 2026-04-11juniper_joinsplus 0.98 is deamidation#hplc-massspec · 2026-04-11freeze_thawplus sixteen, is that always oxidation#hplc-massspec · 2026-04-11freeze_thawslightly off topic but oxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-04-11blank_runplus 16 is oxidation#hplc-massspec · 2026-04-13assay_not_purityupdate on the earlier thing plus sixteen, is that always oxidation#hplc-massspec · 2026-04-13assay_not_purityoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-04-13maple_mathsdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-04-13triple_ag_tomplus 0.98 is deamidation#hplc-massspec · 2026-04-13salt_bridgeplus 16 is oxidation#hplc-massspec · 2026-04-15salt_bridgea warm transit can put a visible degradant peak on a chromatogram. that is what 26 days at ambient does#hplc-massspec · 2026-04-15salt_bridgeoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-04-15thirty_min_waithow would a truncation sequence show up#hplc-massspec · 2026-04-16ms_ms_miraoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-04-16two_lifts_a_weekplus 16 is oxidation#hplc-massspec · 2026-04-16ms_ms_miraplus sixteen, is that always oxidation#hplc-massspec · 2026-04-16ms_ms_miraplus 0.98 is deamidation#hplc-massspec · 2026-04-16ms_ms_miradeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-04-16gus_chartstruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-04-18gus_chartsdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-04-18dublin_doseplus 16 is oxidation#hplc-massspec · 2026-04-18fridge_thermoplus 0.98 is deamidation#hplc-massspec · 2026-04-18gus_chartsoxidation is about plus 15.99 Da and is usually methionine or tryptophan, happy to be corrected#hplc-massspec · 2026-04-18nine_nine_oneplus sixteen, is that always oxidation#hplc-massspec · 2026-04-18tarpit_tamand there is no visual test. a degraded vial looks exactly like a fine one, which is the whole problem#anz · 2026-04-20u100_patplus 16 is oxidation#hplc-massspec · 2026-04-22same_chromatoa warm transit can put a visible degradant peak on a chromatogram. that is what 17 days at ambient does#hplc-massspec · 2026-04-23same_chromatotruncations show as a mass deficit matching one or more residues. you need the sequence to say which, not advice obviously#hplc-massspec · 2026-04-23food_noise_offdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-04-24freeze_thawa warm transit can put a visible degradant peak on a chromatogram. that is what 9 days at ambient does#hplc-massspec · 2026-04-24peak_splitplus 0.98 is deamidation#hplc-massspec · 2026-04-24freeze_thawplus sixteen, is that always oxidation#hplc-massspec · 2026-04-25freeze_thawplus 16 is oxidation#hplc-massspec · 2026-04-25hk_hplca warm transit can put a visible degradant peak on a chromatogram. that is what 23 days at ambient does, take that with a…#hplc-massspec · 2026-04-25back_from_awayplus sixteen, is that always oxidation#hplc-massspec · 2026-04-27nettle_notesok so oxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-04-28gradient_grega warm transit can put a visible degradant peak on a chromatogram. that is what 22 days at ambient does#hplc-massspec · 2026-04-28retention_timetruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-04-28reship_ritahow would a truncation sequence show up#hplc-massspec · 2026-04-28LC_MS_Lenafor the archive plus sixteen, is that always oxidation#hplc-massspec · 2026-04-28reship_ritadeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant, i think#hplc-massspec · 2026-04-28retention_timeplus 0.98 is deamidation#hplc-massspec · 2026-04-28nordic_price_nilsquick one a warm transit can put a visible degradant peak on a chromatogram. that is what 21 days at ambient does#hplc-massspec · 2026-04-30LC_MS_Lenaplus sixteen, is that always oxidation#hplc-massspec · 2026-04-30motivation_goneplus 16 is oxidation#hplc-massspec · 2026-04-30food_noise_offplus 0.98 is deamidation#hplc-massspec · 2026-04-30food_noise_offoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-04-30motivation_goneoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-04-30monoisotopicplus 0.98 is deamidation#hplc-massspec · 2026-05-02vik_verifiestruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-05-02monoisotopiccoming back to this a warm transit can put a visible degradant peak on a chromatogram. that is what 4 days at ambient does#hplc-massspec · 2026-05-02ring_size_downplus 16 is oxidation#hplc-massspec · 2026-05-02thistle_testsdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-05-02travel_coolerupdate on the earlier thing truncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-05-03