Topic
Degradants
Page 13 of 13 — Deamidation at plus one dalton, oxidation at plus sixteen, truncation sequences, and what a warm transit actually does.
Messages — page 13 of 13
nordic_price_nilshow would a truncation sequence show up#hplc-massspec · 2026-06-17monoisotopicdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-06-18monoisotopicright so a warm transit can put a visible degradant peak on a chromatogram. that is what 6 days at ambient does#hplc-massspec · 2026-06-18karl_fischerright so oxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-06-18chlorhextruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-06-18border_force_bexhow would a truncation sequence show up#hplc-massspec · 2026-06-19drawup_deedeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-06-19related_subsplus 0.98 is deamidation#hplc-massspec · 2026-06-20related_subsplus 16 is oxidation#hplc-massspec · 2026-06-20cat_on_the_trayplus 16 is oxidation#hplc-massspec · 2026-06-23pinch_not_stretcha warm transit can put a visible degradant peak on a chromatogram. that is what 26 days at ambient does, i think#hplc-massspec · 2026-06-26pinch_not_stretchoxidation is about plus 15.99 Da and is usually methionine or tryptophan, ill dig out the number#hplc-massspec · 2026-06-26acetate_ashright so plus sixteen, is that always oxidation#hplc-massspec · 2026-06-26noct.titrateplus 16 is oxidation#hplc-massspec · 2026-06-26acetate_ashgenuine question deamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant, happy to be corrected#hplc-massspec · 2026-06-26noct.titratehow would a truncation sequence show up#hplc-massspec · 2026-06-26half_life_halplus 0.98 is deamidation#hplc-massspec · 2026-06-26charge_statetruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-06-27glucagon_armplus sixteen, is that always oxidation#hplc-massspec · 2026-06-27void_volumehow would a truncation sequence show up#hplc-massspec · 2026-06-27void_volumea warm transit can put a visible degradant peak on a chromatogram. that is what 18 days at ambient does#hplc-massspec · 2026-06-27void_volumedeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-06-27lot_number_louoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-06-28fasting_insulinplus sixteen, is that always oxidation#hplc-massspec · 2026-06-28private_script_priplus 16 is oxidation#hplc-massspec · 2026-06-28fasting_insulinplus 0.98 is deamidation#hplc-massspec · 2026-06-28logrotatetruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-06-30logrotateoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-06-30