#hplc-massspec 2026-04-27

Monday14 messages3 participantstimes are UTC

Highlights from this day

back_from_away — quick one ms/ms sequencing is the only thing i would call identity. everything else is circumstantial 14:01
marrow.mod — coming back to this retention time is a hint, not a fingerprint. two different peptides can co-elute perfectly happily 15:17
back_from_away — if somebody says they ran a mass spec and does not show you a spectrum, that is a claim, not a result 15:19
marrow.mod — for the archive can you tell a dimer from the chromatogram alone 16:20
marrow.mod — if the number you are staring at is a third of what you expected, do the charge state arithmetic before you panic, n of 1 obviously 16:24

quick one ms/ms sequencing is the only thing i would call identity. everything else is circumstantial

retention time is a hint

coming back to this retention time is a hint, not a fingerprint. two different peptides can co-elute perfectly happily

run a blank

if somebody says they ran a mass spec and does not show you a spectrum, that is a claim, not a result

plus sixteen, is that always oxidation

shallow gradient

✅2😂9

for the archive can you tell a dimer from the chromatogram alone

👍18🤝3

baseline drift on a gradient is normal. baseline drift on an isocratic hold is your column

[edited]

if the number you are staring at is a third of what you expected, do the charge state arithmetic before you panic, n of 1 obviously

transit-times.csv

169 rows · not retained in the public archive

is uv at 214 or 280 better for peptides

slightly off topic but how do you tell co-elution from a clean single peak

how do you spot a deletion in a sequence from mass alone

within inter-lab range