Topic
Degradants
Page 9 of 13 — Deamidation at plus one dalton, oxidation at plus sixteen, truncation sequences, and what a warm transit actually does.
Messages — page 9 of 13
LC_MS_Lenaoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-03-04patent_2026oxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-03-06patent_2026plus 0.98 is deamidation#hplc-massspec · 2026-03-06salt_bridgehow would a truncation sequence show up#hplc-massspec · 2026-03-06salt_bridgetruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-03-06hk_hplcplus 0.98 is deamidation#hplc-massspec · 2026-03-08plus_sixteendeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-03-08hk_hplctruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-03-08peak_splita warm transit can put a visible degradant peak on a chromatogram. that is what 14 days at ambient does#hplc-massspec · 2026-03-08aliquot_adaplus sixteen, is that always oxidation#hplc-massspec · 2026-03-11gradient_gregok so a warm transit can put a visible degradant peak on a chromatogram. that is what 9 days at ambient does#hplc-massspec · 2026-03-11aliquot_adahow would a truncation sequence show up#hplc-massspec · 2026-03-11lyophile_livok so oxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-03-12coelution_caihow would a truncation sequence show up#hplc-massspec · 2026-03-12lyophile_livright so a warm transit can put a visible degradant peak on a chromatogram. that is what 6 days at ambient does#hplc-massspec · 2026-03-12lyophile_livplus 16 is oxidation#hplc-massspec · 2026-03-12gradient_gregplus 0.98 is deamidation#hplc-massspec · 2026-03-13stack_sceptictruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-03-13gradient_gregfor the archive truncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-03-14no_appetite_niaunrelated but deamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-03-14split_dose_samtruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-03-18still_here_2024which produces the endless it is 2 Da off, is it degraded panic#hplc-massspec · 2026-03-18logrotate5 Da apart on a 3+ ion means about 15 or 16 Da apart on the neutral. that is the oxidation signature#hplc-massspec · 2026-03-18ghent_gradienta warm transit can put a visible degradant peak on a chromatogram. that is what 26 days at ambient does, i think#hplc-massspec · 2026-03-23two_four_ceilingupdate on the earlier thing truncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-03-23tail_factorplus 16 is oxidation#hplc-massspec · 2026-03-24freeze_thawhow would a truncation sequence show up#hplc-massspec · 2026-03-24salt_bridgeoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-03-24yara_yieldsa warm transit can put a visible degradant peak on a chromatogram. that is what 13 days at ambient does#hplc-massspec · 2026-03-24salt_bridgewhile im here plus sixteen, is that always oxidation#hplc-massspec · 2026-03-27salt_bridgea warm transit can put a visible degradant peak on a chromatogram. that is what 19 days at ambient does#hplc-massspec · 2026-03-27acetate_ashgenuine question deamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-03-27lisbon_lottruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-03-27truncation_truplus 0.98 is deamidation#hplc-massspec · 2026-03-28low_and_slowplus 16 is oxidation#hplc-massspec · 2026-03-28low_and_slowtruncations show as a mass deficit matching one or more residues. you need the sequence to say which relative retention time…#hplc-massspec · 2026-03-28dexa_or_biaoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-03-28dexa_or_biaa warm transit can put a visible degradant peak on a chromatogram. that is what 15 days at ambient does#hplc-massspec · 2026-03-28first_month_fihow would a truncation sequence show up#hplc-massspec · 2026-03-28first_month_fiunrelated but deamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant, ymmv#hplc-massspec · 2026-03-28cleo_testsplus sixteen, is that always oxidation#hplc-massspec · 2026-03-28assay_not_purityusually yes. known degradants behave predictably. unknowns are unknowns#coa-reading · 2026-03-29apob_over_ldltruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-03-29apob_over_ldlhow would a truncation sequence show up#hplc-massspec · 2026-03-29month_six_meoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-03-30month_six_meupdate on the earlier thing plus sixteen, is that always oxidation#hplc-massspec · 2026-03-30month_six_mehow would a truncation sequence show up#hplc-massspec · 2026-03-30month_six_meplus 0.98 is deamidation#hplc-massspec · 2026-03-30marrow.modplus 16 is oxidation#hplc-massspec · 2026-03-30void_volumetruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-03-30snac_and_waterplus 16 is oxidation#hplc-massspec · 2026-03-31month_six_megenuine question truncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-03-31taper_tessa warm transit can put a visible degradant peak on a chromatogram. that is what 7 days at ambient does, from memory#hplc-massspec · 2026-04-02ghent_gradientoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-04-04vik_verifieshow would a truncation sequence show up#hplc-massspec · 2026-04-04pharmac_penplus 0.98 is deamidation#hplc-massspec · 2026-04-04pharmac_penplus 16 is oxidation#hplc-massspec · 2026-04-04triple_ag_tomtruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-04-04step_one_siantruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-04-05quiet.hoursa warm transit can put a visible degradant peak on a chromatogram. that is what 23 days at ambient does#hplc-massspec · 2026-04-05