Topic
Degradants
Page 8 of 13 — Deamidation at plus one dalton, oxidation at plus sixteen, truncation sequences, and what a warm transit actually does.
Messages — page 8 of 13
void_volumedeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-02-02dublin_doseoxidation is about plus 15.99 Da and is usually methionine or tryptophan, i have it written down somewhere#hplc-massspec · 2026-02-02tail_factorplus 0.98 is deamidation#hplc-massspec · 2026-02-02oasis_olahow would a truncation sequence show up#hplc-massspec · 2026-02-02knees_firstoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-02-06deamidationplus sixteen, is that always oxidation#hplc-massspec · 2026-02-06deamidationa warm transit can put a visible degradant peak on a chromatogram. that is what 16 days at ambient does truncations show as a…#hplc-massspec · 2026-02-06ms_ms_miraplus sixteen, is that always oxidation#hplc-massspec · 2026-02-08HPLC_Hankdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-02-08zoya_zeroshow would a truncation sequence show up semaglutide is about 4113.6 Da. [M+2H]2+ lands near 2057.8, [M+3H]3+ near 1372.2#hplc-massspec · 2026-02-08assay_not_purityoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-02-08triple_ag_tomplus 16 is oxidation#hplc-massspec · 2026-02-08triple_ag_toma warm transit can put a visible degradant peak on a chromatogram. that is what 11 days at ambient does#hplc-massspec · 2026-02-08pioneer_piaplus 0.98 is deamidation#hplc-massspec · 2026-02-08HPLC_Hankhow would a truncation sequence show up#hplc-massspec · 2026-02-09first_and_last_fouroxidation is about plus 15.99 Da and is usually methionine or tryptophan, i could be wrong#hplc-massspec · 2026-02-09cold_chain_cmdrfollow up deamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-02-10LC_MS_Lenaa warm transit can put a visible degradant peak on a chromatogram. that is what 9 days at ambient does, i could be wrong#hplc-massspec · 2026-02-10LC_MS_Lenaplus 0.98 is deamidation#hplc-massspec · 2026-02-10igf_one_ivyoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-02-12salt_bridgeplus 16 is oxidation#hplc-massspec · 2026-02-12salt_bridgeplus 0.98 is deamidation#hplc-massspec · 2026-02-12deamidationplus sixteen, is that always oxidation#hplc-massspec · 2026-02-12stack_sceptica warm transit can put a visible degradant peak on a chromatogram. that is what 20 days at ambient does#hplc-massspec · 2026-02-12stack_sceptichow would a truncation sequence show up#hplc-massspec · 2026-02-12area_percentdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-02-12void_volumeright so a warm transit can put a visible degradant peak on a chromatogram. that is what 10 days at ambient does#hplc-massspec · 2026-02-15void_volumecoming back to this truncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-02-15void_volumeplus 0.98 is deamidation#hplc-massspec · 2026-02-15LC_MS_Lenahow would a truncation sequence show up#hplc-massspec · 2026-02-15wren_weighs_inplus 16 is oxidation#hplc-massspec · 2026-02-19retention_timea warm transit can put a visible degradant peak on a chromatogram. that is what 19 days at ambient does#hplc-massspec · 2026-02-19retention_timehow would a truncation sequence show up#hplc-massspec · 2026-02-19retention_timedeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-02-19impersonator_ipplus sixteen, is that always oxidation#hplc-massspec · 2026-02-23deamidationa warm transit can put a visible degradant peak on a chromatogram. that is what 12 days at ambient does#hplc-massspec · 2026-02-23deamidationplus 0.98 is deamidation#hplc-massspec · 2026-02-23ten_of_tenplus 16 is oxidation#hplc-massspec · 2026-02-23salt_bridgequick one oxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-02-25quiet.hourshow would a truncation sequence show up#hplc-massspec · 2026-02-25luerlokplus 16 is oxidation#hplc-massspec · 2026-02-25gip_glp_gila warm transit can put a visible degradant peak on a chromatogram. that is what 6 days at ambient does#hplc-massspec · 2026-02-25salt_bridgetruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-02-25karl_fischerdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-02-25LC_MS_Lenaplus 16 is oxidation#hplc-massspec · 2026-02-28split_dose_samplus 0.98 is deamidation#hplc-massspec · 2026-02-28split_dose_sama warm transit can put a visible degradant peak on a chromatogram. that is what 8 days at ambient does#hplc-massspec · 2026-02-28peak_splitplus sixteen, is that always oxidation#hplc-massspec · 2026-02-28nine_nine_oneslightly off topic but truncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-02-28deamidationtruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-03-01LC_MS_Lenawhile im here oxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-03-01freeze_thawplus 16 is oxidation#hplc-massspec · 2026-03-02area_percentplus sixteen, is that always oxidation#hplc-massspec · 2026-03-02birch_bloodstruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-03-02two_four_ceilinghow would a truncation sequence show up#hplc-massspec · 2026-03-02gradient_grega warm transit can put a visible degradant peak on a chromatogram. that is what 24 days at ambient does#hplc-massspec · 2026-03-02peak_splitdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-03-02coa_or_copeplus 0.98 is deamidation#hplc-massspec · 2026-03-02non_scale_winplus sixteen, is that always oxidation#hplc-massspec · 2026-03-04vat_on_importtruncations show as a mass deficit matching one or more residues. you need the sequence to say which, i think#hplc-massspec · 2026-03-04