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Freezing and freeze-thaw

Page 6 of 15 — The long-running argument about freezing reconstituted peptide, and what freeze-thaw cycling appears to do.

Messages — page 6 of 15

verify_or_dontis a frozen lyophilised vial ruined or is that fine#canada · 2025-10-01verify_or_dontpowder survived a freeze#canada · 2025-10-01provincial_patwinter shipping into a cold country risks freezing, which is a different problem from heat and gets discussed less#shipping-customs · 2025-10-02sasha_stacksfreeze debate again#storage-coldchain · 2025-10-03residual_solventnot the freezer#storage-coldchain · 2025-10-03residual_solventfreeze thaw cycling is the risk, not one freeze. that is my read of it anyway#storage-coldchain · 2025-10-03provincial_patfreezing reconstituted peptide is the one thing this channel has never agreed on#storage-coldchain · 2025-10-04LC_MS_Lenafreeze debate again#storage-coldchain · 2025-10-04noct.titratea liquid cold pack tells you it was above freezing, not that the vial cooked. lyophilised powder is robust#shipping-customs · 2025-10-04quiet.hoursa liquid cold pack tells you it was above freezing, not that the vial cooked. lyophilised powder is robust#shipping-customs · 2025-10-04clothes_size_cassa liquid cold pack tells you it was above freezing, not that the vial cooked. lyophilised powder is robust#shipping-customs · 2025-10-05van_isle_viala frozen and thawed reconstituted vial is the one thing i would not use, and i cannot point you at a study for that#canada · 2025-10-05reta_resting_hrfreezing not cooking#canada · 2025-10-05reta_resting_hrhas anyone had a vial actually freeze in transit#canada · 2025-10-05typosquat_taythe winter risk in canada is freezing rather than cooking, and this channel took a while to internalise that, happy to be…#canada · 2025-10-05drawup_deegenuine question freeze thaw cycling is the risk, not one freeze. that is my read of it anyway two to eight degrees. most…#storage-coldchain · 2025-10-08chlorhexquick one a liquid cold pack tells you it was above freezing, not that your vial cooked#storage-coldchain · 2025-10-08chlorhexi freeze aliquots and have had them test fine afterwards. others here would not do it and i understand why#storage-coldchain · 2025-10-08apnoea_down_twofreezing reconstituted peptide is the one thing this channel has never agreed on#storage-coldchain · 2025-10-08tallinn_taperwinter shipping, is freezing the actual risk rather than heat#canada · 2025-10-08verify_or_donta frozen and thawed reconstituted vial is the one thing i would not use, and i cannot point you at a study for that#canada · 2025-10-08pin29gupdate on the earlier thing the winter risk in canada is freezing rather than cooking, and this channel took a while to…#canada · 2025-10-08fridge_thermofreeze thaw cycling is the concern rather than one freeze, and that is my read of it rather than a lab result#canada · 2025-10-08deamidationcoming back to this a liquid cold pack tells you it was above freezing, not that your vial cooked, for what its worth#storage-coldchain · 2025-10-10mg_per_mlnot the freezer#storage-coldchain · 2025-10-10endotoxin_edfreeze thaw cycling is the risk, not one freeze. that is my read of it anyway, i could be wrong#storage-coldchain · 2025-10-11back_from_awaynot the freezer#storage-coldchain · 2025-10-11protein_floordoes anyone freeze reconstituted vials or is that madness#storage-coldchain · 2025-10-11back_from_awayhow cold is too cold, can it actually freeze in a normal fridge#storage-coldchain · 2025-10-11hot_mailboxi freeze aliquots and have had them test fine afterwards. others here would not do it and i understand why#storage-coldchain · 2025-10-11deamidationfreezing reconstituted peptide is the one thing this channel has never agreed on#storage-coldchain · 2025-10-12half_life_halfreeze thaw cycling is the risk, not one freeze. that is my read of it anyway#storage-coldchain · 2025-10-12pbs_pipinsulated pouch, gel packs frozen solid, fastest leg you can buy. that is the whole summer protocol as this room has it#anz · 2025-10-12invoice_mismatchfreeze thaw cycling is the risk, not one freeze. that is my read of it anyway, i could be wrong#storage-coldchain · 2025-10-14glasgow_vialnot the freezer#storage-coldchain · 2025-10-14abdo_two_inchfreeze thaw cycling is the risk, not one freeze. that is my read of it anyway#storage-coldchain · 2025-10-16bea_reconstituteshow cold is too cold, can it actually freeze in a normal fridge#storage-coldchain · 2025-10-16cold_chain_cmdrfreezing reconstituted peptide is the one thing this channel has never agreed on#storage-coldchain · 2025-10-17ferritin_fayfor the archive has anyone had a vial actually freeze in transit#canada · 2025-10-17septum_salfreezing reconstituted peptide is the one thing this channel has never agreed on#storage-coldchain · 2025-10-18electrolyte_elia liquid cold pack tells you it was above freezing, not that your vial cooked i left a reconstituted vial out overnight once,…#storage-coldchain · 2025-10-18fridge_thermothe winter risk in canada is freezing rather than cooking, and this channel took a while to internalise that#canada · 2025-10-18fridge_thermoa frozen and thawed reconstituted vial is the one thing i would not use, and i cannot point you at a study for that#canada · 2025-10-19reship_ritawinter shipping into a cold country risks freezing, which is a different problem from heat and gets discussed less#shipping-customs · 2025-10-21cricket_or_footyinsulated pouch, gel packs frozen solid, fastest leg you can buy. that is the whole summer protocol as this room has it, ill…#anz · 2025-10-21hot_mailboxhow cold is too cold, can it actually freeze in a normal fridge#storage-coldchain · 2025-10-22deamidationa liquid cold pack tells you it was above freezing, not that your vial cooked the vial that visibly changed after a warm…#storage-coldchain · 2025-10-22plus_sixteenthe practical risk in winter shipping is freezing, and in summer it is heat. plan for the season you are in#storage-coldchain · 2025-10-22verify_or_dontthe winter risk in canada is freezing rather than cooking, and this channel took a while to internalise that#canada · 2025-10-22manila_meniscusa liquid cold pack tells you it was above freezing, not that the vial cooked. lyophilised powder is robust#shipping-customs · 2025-10-24orfor_watchi freeze aliquots and have had them test fine afterwards. others here would not do it and i understand why#storage-coldchain · 2025-10-26a1c_lagupdate on the earlier thing a liquid cold pack tells you it was above freezing, not that your vial cooked a few hours at room…#storage-coldchain · 2025-10-26blind_submitdoes anyone freeze reconstituted vials or is that madness#storage-coldchain · 2025-10-26fridge_thermois a frozen lyophilised vial ruined or is that fine#canada · 2025-10-26reta_resting_hrfreeze thaw cycling is the concern rather than one freeze, and that is my read of it rather than a lab result#canada · 2025-10-27marrow.modwinter shipping into a cold country risks freezing, which is a different problem from heat and gets discussed less#shipping-customs · 2025-10-28luerloki cook normally and freeze it in small pots. the food is unchanged, the portion is the only difference#food-and-training · 2025-10-28underfill_umaa liquid cold pack tells you it was above freezing, not that your vial cooked#storage-coldchain · 2025-10-30underfill_umathe practical risk in winter shipping is freezing, and in summer it is heat. plan for the season you are in#storage-coldchain · 2025-10-30per_mg_petehow cold is too cold, can it actually freeze in a normal fridge#storage-coldchain · 2025-10-31