thats fine
#reconstitution 2026-04-03
Friday60 messages13 participantstimes are UTC
Highlights from this day
- ten_of_ten — genuine question what do you do with the last 8 units when you cant draw them cleanly 20:56
- VialBot — Archive lookup: mg_per_ml first appears in this channel on 2026-02-27. 21:10
- VialBot — New independent result logged — QST, lot H-2814, purity 97.4% (VendorInvestigate). 21:40
- hydrate_hana — pick the volume so your dose is a whole number of units. thats the entire trick 22:41
how much do i lose to dead space with a 29G pin
write the date on
too dilute imo
lyophilised powder is far more robust than people think. it is the solution that is fragile
nope, redo it
label the date too. you will not remember which vial is which by week 7, for what its worth
for the archive took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience, not advice obviously
[edited]thats the vacuum
bac water
a slightly collapsed cake is cosmetic. a puddle in the bottom is not
10mg vial
+ 1.0ml -> 10.0 mg/ml 0.5mg = 5u 1mg = 10u
+ 2.0ml -> 5.0 mg/ml 0.5mg = 10u 1mg = 20u
+ 2.5ml -> 4.0 mg/ml 0.5mg = 12.5u (dont)
+ 5.0ml -> 2.0 mg/ml 0.5mg = 25u 1mg = 50uyep 10 units
genuine question what do you do with the last 8 units when you cant draw them cleanly
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i was wrong about this last year: it is fine to draw cold, it just stings more going in, anyway
the jewellery scale is a good habit but calibrate it, they drift badly
clear or bin it
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
Archive lookup: mg_per_ml first appears in this channel on 2026-02-27.
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10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100
read it at eye level
30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
dead space
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
label the vial
round numbers
is there any reason not to just always use 2ml
is it worth buying a 3ml syringe for the diluent or is the insulin one fine
[edited]New independent result logged — QST, lot H-2814, purity 97.4% (VendorInvestigate).
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weigh it first
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
not sterile water
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
the powder is stuck to the stopper, do i care
check your maths
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thats 5mg/ml
give it 5 minutes at room temperature before you decide it has not dissolved
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update on the earlier thing one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
i use bac water even for a single-use vial because it stings less, which is not the reason it exists
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weighing the vial before you reconstitute is the only way to catch an underfill
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can i add more bac water later to a vial ive already reconstituted
i swirl for about thirty seconds and then leave it. never had one fail to dissolve
bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
swirl dont shake
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
update on the earlier thing it should be completely clear with no floaters. cloudy means stop
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sanity check my maths before i pin myself with something stupid
Assay note: SGN lot D-0718 reported at 99% of label content.
i write the mg/ml on the vial with a sharpie the moment i reconstitute it
my rule is one concentration for everything so i never have to think about it at 7am
pick the volume so your dose is a whole number of units. thats the entire trick
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the meniscus thing is real. read it at eye level or you are guessing by a unit either way
unrelated but 10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use, take that with a pinch of salt
temperature of the diluent does not matter. how fast you push it in does
does temperature of the bac water matter at all
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if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space
15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it
dead space in a standard insulin syringe hub is small but it is not nothing over a month, ill dig out the number
aim the diluent at the glass wall, not at the powder. it goes in gentler
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