is the meniscus reading actually a real source of error or am i overthinking
[edited]#reconstitution 2026-01-24
Saturday67 messages13 participantstimes are UTC
Highlights from this day
- stable_not_volatile — 30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right 12:58
- lyophile_liv — a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with 14:23
- sharps_bin_sid — the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol 15:07
- first_and_last_four — do i swirl for a specific length of time or just until its clear 17:52
right so my vial pulled the water in on its own, is that the vacuum or did i do something
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
dead space
On this day 8 years ago this channel logged 132 messages.
bac water or sterile water for something im going into weekly for 3 weeks
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
thats 5mg/ml
label the date too. you will not remember which vial is which by week 20
a slightly collapsed cake is cosmetic. a puddle in the bottom is not, we shall see
do you draw up with the same pin you inject with
i write the mg/ml on the vial with a sharpie the moment i reconstitute it
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
update from 26 months ago: switched to a single concentration and have not miscalculated since
round numbers
30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
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check your maths
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bacteriostatic water for anything multi-use. sterile water has no preservative in it at all, i could be wrong
quick one it foamed. is it ruined
gross powder mass is not net peptide. water and counterion are in that number
my 10mg vial weighed 11.4g on the jewellery scale, is that wrong
coming back to this do not add more bac water to an already reconstituted vial, you have lost track of the concentration
i keep a spare vial of bac water in the fridge purely so i never rush the maths, ask me again in a month
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
my rule is one concentration for everything so i never have to think about it at 7am
lyophilised powder is far more robust than people think. it is the solution that is fragile
label the date too. you will not remember which vial is which by week 31
swirl dont shake
is a slightly cloudy solution ever ok or is that always a bin it
weighing the vial before you reconstitute is the only way to catch an underfill
stable_not_volatile30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its…
temperature of the diluent does not matter. how fast you push it in does
aim the diluent at the glass wall, not at the powder. it goes in gentler
dead space in a standard insulin syringe hub is small but it is not nothing over a month
i use bac water even for a single-use vial because it stings less, which is not the reason it exists
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is it normal for the cake to look slightly collapsed on one side
if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
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clear or bin it
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a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
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nope, redo it
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im getting bubbles in the barrel every single time, what am i doing wrong
can i add more bac water later to a vial ive already reconstituted
not sterile water
does it matter if i inject the bac water straight at the powder
ok so 5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
is it worth buying a 3ml syringe for the diluent or is the insulin one fine
too dilute imo
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thats the vacuum
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
sanity check my maths before i pin myself with something stupid
sorry to jump in swirl it. do not shake it. the foam is denatured protein and you are not getting it back
the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol
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whats the smallest volume you would trust yourself to measure on a u100
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one unit is 0.01ml
follow up the jewellery scale is a good habit but calibrate it, they drift badly
foam is bad
read it at eye level
right so it should be completely clear with no floaters. cloudy means stop
write the date on
anyone worked out a recon volume that puts 10mg on a round unit mark
i swirl for about thirty seconds and then leave it. never had one fail to dissolve
if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space
why does everyone say 2ml specifically and not 1ml
do i swirl for a specific length of time or just until its clear
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nobody here should be telling you a dose. we can check your arithmetic, thats a different thing, thats one data point
what do you do with the last 15 units when you cant draw them cleanly
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if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
do you let it come to room temp before drawing or draw it cold
i was wrong about this last year: it is fine to draw cold, it just stings more going in, n of 1 obviously
coming back after 24 months, has the pinned recon volume advice changed