vialroom

#reconstitution 2025-11-27

Thursday33 messages8 participantstimes are UTC
Highlights from this day
  • pharmac_pen — benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically 10:57
  • relabel_watch — if i add 5ml to a 5mg vial what concentration am i actually at 12:51
  • otto_swirls — anyone got a photo of what a properly dissolved vial looks like 12:56
PP

benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically

👍13
GC

is it worth buying a 3ml syringe for the diluent or is the insulin one fine

if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration

LM

a slightly collapsed cake is cosmetic. a puddle in the bottom is not, i have it written down somewhere

PP

do i swirl for a specific length of time or just until its clear

FF

if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
it should be completely clear with no floaters. cloudy means stop

weighing the vial before you reconstitute is the only way to catch an underfill

LM

anyone else find the 10mg vials harder to get fully dissolved than the small ones

the last few units are a real loss. i accept it rather than tilting the vial and drawing air

pick the volume so your dose is a whole number of units. thats the entire trick
a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with

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if i add 5ml to a 5mg vial what concentration am i actually at

👀17

the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol

anyone worked out a recon volume that puts 2.4mg on a round unit mark

OS

anyone got a photo of what a properly dissolved vial looks like

10mg vial
  + 1.0ml  -> 10.0 mg/ml   0.5mg =  5u   1mg = 10u
  + 2.0ml  ->  5.0 mg/ml   0.5mg = 10u   1mg = 20u
  + 2.5ml  ->  4.0 mg/ml   0.5mg = 12.5u (dont)
  + 5.0ml  ->  2.0 mg/ml   0.5mg = 25u   1mg = 50u
OS

sanity check my maths before i pin myself with something stupid

15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it

not sterile water

RW

gross powder mass is not net peptide. water and counterion are in that number, ill dig out the number

one unit is 0.01ml

lyophilised powder is far more robust than people think. it is the solution that is fragile

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nobody here should be telling you a dose. we can check your arithmetic, thats a different thing

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