do you let it come to room temp before drawing or draw it cold
gross powder mass is not net peptide. water and counterion are in that number
#reconstitution 2025-08-28
Thursday27 messages6 participantstimes are UTC
Highlights from this day
- c18_column — do you let it come to room temp before drawing or draw it cold gross powder mass is not net peptide. water and counterion are in that number 09:57
- gall_bladder_gav — if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space, i think 10:47
- gall_bladder_gav — bacteriostatic water for anything multi-use. sterile water has no preservative in it at all, happy to be corrected 14:09
- c18_column — dead space in a standard insulin syringe hub is small but it is not nothing over a month 14:22
- first_vial_finn — if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration, happy to be corrected 14:23
how much do i lose to dead space with a 30G pin
round numbers
15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it
pick the volume so your dose is a whole number of units. thats the entire trick
if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space, i think
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quick one is it normal for the cake to look slightly collapsed on one side
aim the diluent at the glass wall, not at the powder. it goes in gentler, thats one data point
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
give it 3 minutes at room temperature before you decide it has not dissolved, happy to be corrected
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
[edited]label the date too. you will not remember which vial is which by week 4
label the vial
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a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
it foamed. is it ruined
[edited]gradient_gregswirl it. do not shake it. the foam is denatured protein and you are not getting it back
the powder is stuck to the stopper, do i care
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
nobody here should be telling you a dose. we can check your arithmetic, thats a different thing
dead space
nope, redo it
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it should be completely clear with no floaters. cloudy means stop, we shall see
bacteriostatic water for anything multi-use. sterile water has no preservative in it at all, happy to be corrected
what do you do with the last 25 units when you cant draw them cleanly
weigh it first
the jewellery scale is a good habit but calibrate it, they drift badly
dead space in a standard insulin syringe hub is small but it is not nothing over a month
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if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration, happy to be corrected