swirl it. do not shake it. the foam is denatured protein and you are not getting it back
lyophilised powder is far more robust than people think. it is the solution that is fragile
#reconstitution 2025-07-11
Friday16 messages5 participantstimes are UTC
Highlights from this day
- point_two_five — swirl it. do not shake it. the foam is denatured protein and you are not getting it back lyophilised powder is far more robust than people think. it is the solution… 17:40
- monoisotopic — a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with 17:47
- luerlok — 10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use 17:51
- ema_vs_national — one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that i was wrong about this last year: it is fine to draw cold, it just stings more… 18:46
- ema_vs_national — took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience, ymmv 19:25
yep 10 units
too dilute imo
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a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
U-100 syringe: 1 unit = 0.01 ml
10u = 0.10 ml
25u = 0.25 ml
50u = 0.50 ml
100u = 1.00 ml
mg drawn = ml drawn x mg/mlmy rule is one concentration for everything so i never have to think about it at 7am, still working it out
slightly off topic but my vial pulled the water in on its own, is that the vacuum or did i do something
10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
😂15
a slightly collapsed cake is cosmetic. a puddle in the bottom is not
why does everyone say 2ml specifically and not 1ml
aim the diluent at the glass wall, not at the powder. it goes in gentler
the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol, ill dig out the number
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
i was wrong about this last year: it is fine to draw cold, it just stings more going in
weighing the vial before you reconstitute is the only way to catch an underfill
pick the volume so your dose is a whole number of units. thats the entire trick
took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience, ymmv
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*Medutest not the other one
anyone else find the 15mg vials harder to get fully dissolved than the small ones