vialroom

#reconstitution 2025-07-04

Friday43 messages9 participantstimes are UTC
Highlights from this day
  • VialBot — New independent result logged — GL Biochem, lot G-0641, purity 96.8% (PeptideMeter). 15:18
  • thirty_one_g — took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience 15:40
  • blind_submit — update from 3 months ago: switched to a single concentration and have not miscalculated since, ask me again in a month 16:46
  • auckland_aliquot — you can absolutely just always use 2ml, and honestly most of us should, for what its worth 18:27
VV

dead space in a standard insulin syringe hub is small but it is not nothing over a month
nobody here should be telling you a dose. we can check your arithmetic, thats a different thing

VV

the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol, we shall see

sorry to jump in one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that

A1

sorry to jump in aim the diluent at the glass wall, not at the powder. it goes in gentler

BS

anyone got a photo of what a properly dissolved vial looks like

GV

the jewellery scale is a good habit but calibrate it, they drift badly

GV

if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration

BS

the meniscus thing is real. read it at eye level or you are guessing by a unit either way

BS

slightly off topic but my rule is one concentration for everything so i never have to think about it at 7am

AA

a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with, thats just me

AA

thats fine

U-100 syringe: 1 unit = 0.01 ml
  10u = 0.10 ml
  25u = 0.25 ml
  50u = 0.50 ml
 100u = 1.00 ml
mg drawn = ml drawn x mg/ml
VB

New independent result logged — GL Biochem, lot G-0641, purity 96.8% (PeptideMeter).

GV

i have never once regretted using a rounder number and a slightly bigger volume

TO

took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience

📈4🧊1

not sterile water

pick the volume so your dose is a whole number of units. thats the entire trick

i swirl for about thirty seconds and then leave it. never had one fail to dissolve

TO

thats 5mg/ml

medutest-report-sg-1177.pdf
3 pages · 798 KB · not retained in the public archive
3

can i add more bac water later to a vial ive already reconstituted

BS

update from 3 months ago: switched to a single concentration and have not miscalculated since, ask me again in a month

[edited]
🤝5
TO

i use bac water even for a single-use vial because it stings less, which is not the reason it exists

AA

genuine question label the date too. you will not remember which vial is which by week 8

update on the earlier thing i write the mg/ml on the vial with a sharpie the moment i reconstitute it, your mileage will differ

AA

you can absolutely just always use 2ml, and honestly most of us should, for what its worth

📉3🔥1

clear or bin it

slightly off topic but 20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy

check your maths

coming back after 20 months, has the pinned recon volume advice changed