nope, redo it
#reconstitution 2025-02-21
- vienna_vial — update from 16 months ago: switched to a single concentration and have not miscalculated since, thats just me 12:12
- fake_tracking — i write the mg/ml on the vial with a sharpie the moment i reconstitute it 12:30
- vienna_vial — does temperature of the bac water matter at all lyophilised powder is far more robust than people think. it is the solution that is fragile 13:07
- vienna_vial — 10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use if the powder is on the stopper, tap it down before you add anything. it will still dissolve either… 13:28
- nordic_price_nils — right so weighing the vial before you reconstitute is the only way to catch an underfill 14:03
thats the vacuum
not sterile water
update from 16 months ago: switched to a single concentration and have not miscalculated since, thats just me
do not add more bac water to an already reconstituted vial, you have lost track of the concentration
the jewellery scale is a good habit but calibrate it, they drift badly
i write the mg/ml on the vial with a sharpie the moment i reconstitute it
is it normal for the cake to look slightly collapsed on one side
the meniscus thing is real. read it at eye level or you are guessing by a unit either way
my 10mg vial weighed 11.4g on the jewellery scale, is that wrong
too dilute imo
does temperature of the bac water matter at all
lyophilised powder is far more robust than people think. it is the solution that is fragile
is a slightly cloudy solution ever ok or is that always a bin it
it foamed. is it ruined
10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
my vial pulled the water in on its own, is that the vacuum or did i do something
coming back to this sanity check my maths before i pin myself with something stupid
anyone worked out a recon volume that puts 7.5mg on a round unit mark
right so weighing the vial before you reconstitute is the only way to catch an underfill
quick one 15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
does it matter if i inject the bac water straight at the powder
quick one temperature of the diluent does not matter. how fast you push it in does
can i add more bac water later to a vial ive already reconstituted
genuine question what do you do with the last 20 units when you cant draw them cleanly
if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space
bac water
right so whats the smallest volume you would trust yourself to measure on a u100
a slightly collapsed cake is cosmetic. a puddle in the bottom is not
weigh it first