vialroom

#reconstitution 2024-12-14

Saturday32 messages8 participantstimes are UTC
Highlights from this day
  • VialBot — Testing queue: 6 submissions open, 35 awaiting dispatch. 19:15
  • area_percent — bacteriostatic water for anything multi-use. sterile water has no preservative in it at all 19:25
  • area_percent — do i swirl for a specific length of time or just until its clear 20:34
HH

if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way, n of 1 obviously
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that

GG

my 10mg vial weighed 11.4g on the jewellery scale, is that wrong

VB

Testing queue: 6 submissions open, 35 awaiting dispatch.

CP

i was wrong about this last year: it is fine to draw cold, it just stings more going in

give it 6 minutes at room temperature before you decide it has not dissolved

19:24quiet.hours pinned a message to this channel
AP

bacteriostatic water for anything multi-use. sterile water has no preservative in it at all

10mg vial
  + 1.0ml  -> 10.0 mg/ml   0.5mg =  5u   1mg = 10u
  + 2.0ml  ->  5.0 mg/ml   0.5mg = 10u   1mg = 20u
  + 2.5ml  ->  4.0 mg/ml   0.5mg = 12.5u (dont)
  + 5.0ml  ->  2.0 mg/ml   0.5mg = 25u   1mg = 50u
CP

my rule is one concentration for everything so i never have to think about it at 7am

unrelated but why does everyone say 2ml specifically and not 1ml

RR

aim the diluent at the glass wall, not at the powder. it goes in gentler

how long after reconstituting before its actually dissolved

RR

the last few units are a real loss. i accept it rather than tilting the vial and drawing air

NP

update from 5 months ago: switched to a single concentration and have not miscalculated since

round numbers

is there any reason not to just always use 2ml

dead space in a standard insulin syringe hub is small but it is not nothing over a month

[edited]

write the date on

AP

do i swirl for a specific length of time or just until its clear

🎉14👍1📈1
RR

label the date too. you will not remember which vial is which by week 26

AP

swirl it. do not shake it. the foam is denatured protein and you are not getting it back

AP

if the maths gives you 13.7 units, change the volume. do not try to hit 13.7

20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy

coming back to this benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically

RR

nobody here should be telling you a dose. we can check your arithmetic, thats a different thing