thats the solvent peak
#hplc-massspec 2026-06-06
Saturday14 messages5 participantstimes are UTC
Highlights from this day
- pia_plateaus — whats the void volume peak, is that always solvent 15:37
- freeze_thaw — if the lab reports area percent without stating the wavelength, the number is less useful than it looks, we shall see monoisotopic and average diverge more the bigger… 16:39
- hk_hplc — if the number you are staring at is a third of what you expected, do the charge state arithmetic before you panic 17:53
- hk_hplc — if somebody says they ran a mass spec and does not show you a spectrum, that is a claim, not a result 18:06
whats the void volume peak, is that always solvent
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relative retention time against a standard is comparable between labs. absolute minutes are not
plus 16 is oxidation
is there any point running a blank if the sample is the question
i asked Janoshik for the raw trace and they sent it. that is the right answer to that question
if the lab reports area percent without stating the wavelength, the number is less useful than it looks, we shall see
monoisotopic and average diverge more the bigger the molecule. say which one you are quoting
janoshik-a-2601.pdf
2 pages · 889 KB · not retained in the public archive
truncations show as a mass deficit matching one or more residues. you need the sequence to say which, your mileage will differ
if the number you are staring at is a third of what you expected, do the charge state arithmetic before you panic
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unrelated but a shoulder on a peak is information, and usually bad information
if somebody says they ran a mass spec and does not show you a spectrum, that is a claim, not a result
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the honest position is that hobby-level interpretation of a chromatogram is worth something but not much
fridge-temps.csv
445 rows · not retained in the public archive
you need more sample for identity than for purity. plan for that when you post it, i have it written down somewhere
[edited]within inter-lab range