do you trust an area percent from a shallow gradient
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#hplc-massspec 2025-04-28
Monday11 messages3 participantstimes are UTC
Highlights from this day
- LC_MS_Lena — do you trust an area percent from a shallow gradient 18:15
- HPLC_Hank — retention time is a hint, not a fingerprint. two different peptides can co-elute perfectly happily 18:18
- LC_MS_Lena — a dimer usually shows up late and at roughly double the mass. the chromatogram alone will not tell you 18:58
retention time is a hint, not a fingerprint. two different peptides can co-elute perfectly happily
show me the spectrum
while im here i have a number at about a third of what i expected, is that a charge state
does retention time on its own tell you what the compound is
a dimer usually shows up late and at roughly double the mass. the chromatogram alone will not tell you
retention time is a hint
thats a charge state
whats a normal gradient for a peptide this size
coming back after 26 months, what changed in how you read these
plus 0.98 is deamidation
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