i have a number at about a third of what i expected, is that a charge state
#hplc-massspec 2025-03-25
Tuesday11 messages4 participantstimes are UTC
Highlights from this day
- HPLC_Hank — truncations show as a mass deficit matching one or more residues. you need the sequence to say which 13:43
- HPLC_Hank — plus sixteen, is that always oxidation 13:44
- factory_direct_fi — a dimer usually shows up late and at roughly double the mass. the chromatogram alone will not tell you 14:58
ms/ms or it isnt identity
⚠️16🧊3💀8
integration difference
tirzepatide is about 4813.5 Da, so 2+ is near 2407.8 and 3+ near 1605.5
co-elution is the failure mode that a beautiful single peak is most likely to be hiding
you need more sample for identity than for purity. plan for that when you post it
truncations show as a mass deficit matching one or more residues. you need the sequence to say which
baseline is drifting badly, is that the column or the mobile phase
need more sample
[edited]a dimer usually shows up late and at roughly double the mass. the chromatogram alone will not tell you
[edited]