clear or bin it
#reconstitution 2026-05-27
Wednesday58 messages12 participantstimes are UTC
Highlights from this day
- vienna_vial — a slightly collapsed cake is cosmetic. a puddle in the bottom is not 09:05
- van_isle_vial — coming back after 20 months, has the pinned recon volume advice changed 11:53
- van_isle_vial — is the meniscus reading actually a real source of error or am i overthinking 12:59
- secretagogue_sy — the meniscus thing is real. read it at eye level or you are guessing by a unit either way 14:58
how do you all measure a half unit, or do you just not
my rule is one concentration for everything so i never have to think about it at 7am, take that with a pinch of salt
label the vial
anyone worked out a recon volume that puts 1mg on a round unit mark
transit-times.csv
760 rows · not retained in the public archive
do not add more bac water to an already reconstituted vial, you have lost track of the concentration, take that with a pinch of salt
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slightly off topic but i swirl for about thirty seconds and then leave it. never had one fail to dissolve
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
i use bac water even for a single-use vial because it stings less, which is not the reason it exists
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sorry to jump in what do you do with the last 40 units when you cant draw them cleanly
weigh it first
lyophilised powder is far more robust than people think. it is the solution that is fragile
dead space in a standard insulin syringe hub is small but it is not nothing over a month
my 10mg vial weighed 11.4g on the jewellery scale, is that wrong
it should be completely clear with no floaters. cloudy means stop
why does everyone say 2ml specifically and not 1ml
thats fine
swirl it. do not shake it. the foam is denatured protein and you are not getting it back, we shall see
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the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol
how much do i lose to dead space with a 29G pin
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update from 8 months ago: switched to a single concentration and have not miscalculated since
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose, still working it out
gross powder mass is not net peptide. water and counterion are in that number
*2ml not 2.5
my vial pulled the water in on its own, is that the vacuum or did i do something
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Recon calculator: 40mg in 5ml = 10mg/ml.
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does benzyl alcohol matter if im finishing the vial in one go
Recon calculator: 15mg in 1.5ml = 5mg/ml.
not sterile water
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
[edited]one unit is 0.01ml
for the archive 20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
follow up is a slightly cloudy solution ever ok or is that always a bin it
temperature of the diluent does not matter. how fast you push it in does
nobody here should be telling you a dose. we can check your arithmetic, thats a different thing
anyone got a photo of what a properly dissolved vial looks like
label the date too. you will not remember which vial is which by week 10
nope, redo it
coming back after 20 months, has the pinned recon volume advice changed
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whats the smallest volume you would trust yourself to measure on a u100
thats 5mg/ml
is it worth buying a 3ml syringe for the diluent or is the insulin one fine
is the meniscus reading actually a real source of error or am i overthinking
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can i add more bac water later to a vial ive already reconstituted
i write the mg/ml on the vial with a sharpie the moment i reconstitute it, your mileage will differ
bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
i write the mg/ml on the vial with a sharpie the moment i reconstitute it
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if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
lyophilised powder is far more robust than people think. it is the solution that is fragile
too dilute imo
dead space
right so does temperature of the bac water matter at all
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
im getting bubbles in the barrel every single time, what am i doing wrong
right so benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
does it matter if i inject the bac water straight at the powder
right so 15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it
the meniscus thing is real. read it at eye level or you are guessing by a unit either way
U-100 syringe: 1 unit = 0.01 ml
10u = 0.10 ml
25u = 0.25 ml
50u = 0.50 ml
100u = 1.00 ml
mg drawn = ml drawn x mg/mlBatch lookup F-1330: 4 independent reports on file, earliest 2025-11-19.
right so took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience
while im here 10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100