my vial pulled the water in on its own, is that the vacuum or did i do something
#reconstitution 2026-04-09
Thursday27 messages6 participantstimes are UTC
Highlights from this day
- ollie_orders — weighing the vial before you reconstitute is the only way to catch an underfill 18:53
- snac_and_water — what do you do with the last 8 units when you cant draw them cleanly 19:11
- snac_and_water — i keep a spare vial of bac water in the fridge purely so i never rush the maths 19:13
- assay_not_purity — the powder is stuck to the stopper, do i care 19:15
- VialBot — Archive lookup: snac_and_water first appears in this channel on 2025-12-13. 19:38
nope, redo it
follow up if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
[edited]round numbers
i write the mg/ml on the vial with a sharpie the moment i reconstitute it
10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
write the date on
[edited]weighing the vial before you reconstitute is the only way to catch an underfill
❤️8
not sterile water
im getting bubbles in the barrel every single time, what am i doing wrong
what do you do with the last 8 units when you cant draw them cleanly
🙏1🧪16✅1
i keep a spare vial of bac water in the fridge purely so i never rush the maths
🤝6👀8🙏14
whats the smallest volume you would trust yourself to measure on a u100
the powder is stuck to the stopper, do i care
🧊7
Archive lookup: snac_and_water first appears in this channel on 2025-12-13.
swirl dont shake
clear or bin it
coming back after 13 months, has the pinned recon volume advice changed
lyophilised powder is far more robust than people think. it is the solution that is fragile
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
the jewellery scale is a good habit but calibrate it, they drift badly
i use bac water even for a single-use vial because it stings less, which is not the reason it exists
follow up one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
ok so benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically, i have it written down somewhere
do i swirl for a specific length of time or just until its clear
can i add more bac water later to a vial ive already reconstituted