vialroom

#reconstitution 2026-04-05

Sunday37 messages8 participantstimes are UTC
Highlights from this day
  • ida_injects — is there any reason not to just always use 2ml 13:46
  • tail_factor — is the meniscus reading actually a real source of error or am i overthinking 14:48
  • ida_injects — nobody here should be telling you a dose. we can check your arithmetic, thats a different thing, thats one data point 16:28

sanity check my maths before i pin myself with something stupid

is there any reason not to just always use 2ml

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bac water

weigh it first

SN

update from 2 months ago: switched to a single concentration and have not miscalculated since, not advice obviously

thats 5mg/ml

DD

15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it

10mg vial
  + 1.0ml  -> 10.0 mg/ml   0.5mg =  5u   1mg = 10u
  + 2.0ml  ->  5.0 mg/ml   0.5mg = 10u   1mg = 20u
  + 2.5ml  ->  4.0 mg/ml   0.5mg = 12.5u (dont)
  + 5.0ml  ->  2.0 mg/ml   0.5mg = 25u   1mg = 50u
DD

im getting bubbles in the barrel every single time, what am i doing wrong

TF

my 10mg vial weighed 11.4g on the jewellery scale, is that wrong
if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration

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is the meniscus reading actually a real source of error or am i overthinking

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aim the diluent at the glass wall, not at the powder. it goes in gentler

CS

dead space in a standard insulin syringe hub is small but it is not nothing over a month
if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration

DD

sorry to jump in do i swirl for a specific length of time or just until its clear

CS

the meniscus thing is real. read it at eye level or you are guessing by a unit either way

II

swirl it. do not shake it. the foam is denatured protein and you are not getting it back
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that

[edited]

*VendorInvestigate not the other one

nobody here should be telling you a dose. we can check your arithmetic, thats a different thing, thats one data point

10mg vial
  + 1.0ml  -> 10.0 mg/ml   0.5mg =  5u   1mg = 10u
  + 2.0ml  ->  5.0 mg/ml   0.5mg = 10u   1mg = 20u
  + 2.5ml  ->  4.0 mg/ml   0.5mg = 12.5u (dont)
  + 5.0ml  ->  2.0 mg/ml   0.5mg = 25u   1mg = 50u
II

genuine question i was wrong about this last year: it is fine to draw cold, it just stings more going in

VI

coming back after 3 months, has the pinned recon volume advice changed

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17:35marrow.mod pinned a message to this channel
VI

10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use

RL

what do you do with the last 50 units when you cant draw them cleanly

CS

30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right

[edited]
RR

gross powder mass is not net peptide. water and counterion are in that number, from memory

the last few units are a real loss. i accept it rather than tilting the vial and drawing air

dead space