while im here i keep a spare vial of bac water in the fridge purely so i never rush the maths
#reconstitution 2026-03-22
Sunday46 messages10 participantstimes are UTC
Highlights from this day
- customs_owl — whats the smallest volume you would trust yourself to measure on a u100 14:18
- tenpence — swirl it. do not shake it. the foam is denatured protein and you are not getting it back 14:49
- two_three_lifts — took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience, for what its worth 15:41
quick one does temperature of the bac water matter at all
i write the mg/ml on the vial with a sharpie the moment i reconstitute it
*Janoshik not the other one
im getting bubbles in the barrel every single time, what am i doing wrong
whats the smallest volume you would trust yourself to measure on a u100
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*PeptideMeter not the other one
anyone else find the 20mg vials harder to get fully dissolved than the small ones
U-100 syringe: 1 unit = 0.01 ml
10u = 0.10 ml
25u = 0.25 ml
50u = 0.50 ml
100u = 1.00 ml
mg drawn = ml drawn x mg/mlhow long after reconstituting before its actually dissolved
is the meniscus reading actually a real source of error or am i overthinking
my rule is one concentration for everything so i never have to think about it at 7am
15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it
14:47tenpence pinned a message to this channel
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
peptidemeter-summary-f-1330.pdf
2 pages · 767 KB · not retained in the public archive
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gross powder mass is not net peptide. water and counterion are in that number
the meniscus thing is real. read it at eye level or you are guessing by a unit either way, someone check my working
do i swirl for a specific length of time or just until its clear
sanity check my maths before i pin myself with something stupid
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is it normal for the cake to look slightly collapsed on one side
took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience, for what its worth
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5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
[edited]read it at eye level
the last few units are a real loss. i accept it rather than tilting the vial and drawing air, i have it written down somewhere
it should be completely clear with no floaters. cloudy means stop
thats the vacuum
while im here how do you all measure a half unit, or do you just not
it foamed. is it ruined
anyone got a photo of what a properly dissolved vial looks like
dead space
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while im here bac water or sterile water for something im going into weekly for 9 weeks
a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
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17:06tenpence pinned a message to this channel
slightly off topic but my vial pulled the water in on its own, is that the vacuum or did i do something
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thats 5mg/ml
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do you let it come to room temp before drawing or draw it cold
i use bac water even for a single-use vial because it stings less, which is not the reason it exists
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30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
weighing the vial before you reconstitute is the only way to catch an underfill
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what do you do with the last 25 units when you cant draw them cleanly
label the date too. you will not remember which vial is which by week 8
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
weighing the vial before you reconstitute is the only way to catch an underfill
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
does it matter if i inject the bac water straight at the powder
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
is it worth buying a 3ml syringe for the diluent or is the insulin one fine
nope, redo it
check your maths