vialroom

#reconstitution 2026-03-19

Thursday36 messages7 participantstimes are UTC
Highlights from this day
  • surpass_two — do i swirl for a specific length of time or just until its clear 18:07
  • u100_pat — anyone worked out a recon volume that puts 2.5mg on a round unit mark took me a year to stop over-diluting. the measurement error at 4 units is worse than the… 20:19
  • u100_pat — bacteriostatic water for anything multi-use. sterile water has no preservative in it at all, someone check my working 20:32
  • u100_pat — gross powder mass is not net peptide. water and counterion are in that number 20:35
  • mg_per_ml — the jewellery scale is a good habit but calibrate it, they drift badly 20:51
EM

update on the earlier thing 5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose, we shall see

WW

if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way

anyone got a photo of what a properly dissolved vial looks like

ST

do i swirl for a specific length of time or just until its clear

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ST

10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100

ST

a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with

ST

swirl it. do not shake it. the foam is denatured protein and you are not getting it back

SS

while im here does benzyl alcohol matter if im finishing the vial in one go

MP

15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it

dead space

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nobody here should be telling you a dose. we can check your arithmetic, thats a different thing

i swirl for about thirty seconds and then leave it. never had one fail to dissolve

U1

anyone else find the 15mg vials harder to get fully dissolved than the small ones

EM

foam is bad

coa-a-2418.pdf
1 page · 162 KB · not retained in the public archive

slightly off topic but aim the diluent at the glass wall, not at the powder. it goes in gentler

U1

anyone worked out a recon volume that puts 2.5mg on a round unit mark
took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience

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i have never once regretted using a rounder number and a slightly bigger volume

U1

i write the mg/ml on the vial with a sharpie the moment i reconstitute it

bacteriostatic water for anything multi-use. sterile water has no preservative in it at all, someone check my working

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label the date too. you will not remember which vial is which by week 9

gross powder mass is not net peptide. water and counterion are in that number

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slightly off topic but i keep a spare vial of bac water in the fridge purely so i never rush the maths

MP

the last few units are a real loss. i accept it rather than tilting the vial and drawing air

MP

took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience

sorry to jump in dead space in a standard insulin syringe hub is small but it is not nothing over a month

the jewellery scale is a good habit but calibrate it, they drift badly

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