i swirl for about thirty seconds and then leave it. never had one fail to dissolve
#reconstitution 2026-03-01
Sunday46 messages12 participantstimes are UTC
Highlights from this day
- amylin_amy — quick one do not add more bac water to an already reconstituted vial, you have lost track of the concentration 11:56
- VialBot — Assay note: WXT lot B-0114 reported at 99% of label content. 13:51
- amsterdam_aliquot — swirl it. do not shake it. the foam is denatured protein and you are not getting it back 13:54
- amsterdam_aliquot — i write the mg/ml on the vial with a sharpie the moment i reconstitute it 13:59
- forty_five_kg — genuine question whats the smallest volume you would trust yourself to measure on a u100 16:54
label the date too. you will not remember which vial is which by week 10
lyophilised powder is far more robust than people think. it is the solution that is fragile, thats just me
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bac water or sterile water for something im going into weekly for 8 weeks
how do you all measure a half unit, or do you just not
a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
quick one do not add more bac water to an already reconstituted vial, you have lost track of the concentration
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weigh it first
10mg vial
+ 1.0ml -> 10.0 mg/ml 0.5mg = 5u 1mg = 10u
+ 2.0ml -> 5.0 mg/ml 0.5mg = 10u 1mg = 20u
+ 2.5ml -> 4.0 mg/ml 0.5mg = 12.5u (dont)
+ 5.0ml -> 2.0 mg/ml 0.5mg = 25u 1mg = 50u👀1🙏13
while im here does temperature of the bac water matter at all
is the meniscus reading actually a real source of error or am i overthinking
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round numbers
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anyone got a photo of what a properly dissolved vial looks like
thats the vacuum
transit-times.csv
539 rows · not retained in the public archive
On this day 4 years ago this channel logged 20 messages.
is it normal for the cake to look slightly collapsed on one side
foam is bad
10mg vial
+ 1.0ml -> 10.0 mg/ml 0.5mg = 5u 1mg = 10u
+ 2.0ml -> 5.0 mg/ml 0.5mg = 10u 1mg = 20u
+ 2.5ml -> 4.0 mg/ml 0.5mg = 12.5u (dont)
+ 5.0ml -> 2.0 mg/ml 0.5mg = 25u 1mg = 50u🤝5💀1
anyone worked out a recon volume that puts 1mg on a round unit mark
my rule is one concentration for everything so i never have to think about it at 7am
the meniscus thing is real. read it at eye level or you are guessing by a unit either way
update from 18 months ago: switched to a single concentration and have not miscalculated since, i could be wrong
Assay note: WXT lot B-0114 reported at 99% of label content.
im getting bubbles in the barrel every single time, what am i doing wrong
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
U-100 syringe: 1 unit = 0.01 ml
10u = 0.10 ml
25u = 0.25 ml
50u = 0.50 ml
100u = 1.00 ml
mg drawn = ml drawn x mg/mlnot sterile water
give it 4 minutes at room temperature before you decide it has not dissolved
i write the mg/ml on the vial with a sharpie the moment i reconstitute it
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you can absolutely just always use 2ml, and honestly most of us should
my vial pulled the water in on its own, is that the vacuum or did i do something
unrelated but i have never once regretted using a rounder number and a slightly bigger volume
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if i add 1ml to a 15mg vial what concentration am i actually at
pick the volume so your dose is a whole number of units. thats the entire trick
sanity check my maths before i pin myself with something stupid
read it at eye level
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
the jewellery scale is a good habit but calibrate it, they drift badly
nobody here should be telling you a dose. we can check your arithmetic, thats a different thing
can i add more bac water later to a vial ive already reconstituted
genuine question whats the smallest volume you would trust yourself to measure on a u100
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clear or bin it
do you draw up with the same pin you inject with
is there any reason not to just always use 2ml
i was wrong about this last year: it is fine to draw cold, it just stings more going in
aim the diluent at the glass wall, not at the powder. it goes in gentler
bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
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check your maths