30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
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#reconstitution 2026-02-12
Thursday51 messages10 participantstimes are UTC
Highlights from this day
- VialBot — Verification log updated: FGP — evidence added, status unchanged. 19:09
- quiet_head_qi — update from 24 months ago: switched to a single concentration and have not miscalculated since 19:42
- amsterdam_aliquot — gross powder mass is not net peptide. water and counterion are in that number 19:48
- amsterdam_aliquot — give it 5 minutes at room temperature before you decide it has not dissolved, ymmv 19:51
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
*that should say weekly
clear or bin it
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Verification log updated: FGP — evidence added, status unchanged.
slightly off topic but the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol
quick one a slightly collapsed cake is cosmetic. a puddle in the bottom is not
bac water or sterile water for something im going into weekly for 2 weeks
what do you do with the last 25 units when you cant draw them cleanly
i was wrong about this last year: it is fine to draw cold, it just stings more going in
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took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience, ymmv
10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
round numbers
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
update from 24 months ago: switched to a single concentration and have not miscalculated since
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swirl dont shake
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genuine question you can absolutely just always use 2ml, and honestly most of us should
gross powder mass is not net peptide. water and counterion are in that number
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label the date too. you will not remember which vial is which by week 10
is the meniscus reading actually a real source of error or am i overthinking
give it 5 minutes at room temperature before you decide it has not dissolved, ymmv
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swirl it. do not shake it. the foam is denatured protein and you are not getting it back
bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
my 10mg vial weighed 11.4g on the jewellery scale, is that wrong
pick the volume so your dose is a whole number of units. thats the entire trick
im getting bubbles in the barrel every single time, what am i doing wrong
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*VendorInvestigate not the other one
weighing the vial before you reconstitute is the only way to catch an underfill
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anyone worked out a recon volume that puts 10mg on a round unit mark
how long after reconstituting before its actually dissolved
my rule is one concentration for everything so i never have to think about it at 7am
dead space in a standard insulin syringe hub is small but it is not nothing over a month
not sterile water
can i add more bac water later to a vial ive already reconstituted
does temperature of the bac water matter at all
while im here lyophilised powder is far more robust than people think. it is the solution that is fragile
[edited]bac water
is a slightly cloudy solution ever ok or is that always a bin it
check your maths
if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
the jewellery scale is a good habit but calibrate it, they drift badly
i write the mg/ml on the vial with a sharpie the moment i reconstitute it
label the vial
thats the vacuum
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the powder is stuck to the stopper, do i care
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
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do i swirl for a specific length of time or just until its clear
is there any reason not to just always use 2ml
a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
yep 10 units
foam is bad
my rule is one concentration for everything so i never have to think about it at 7am