vialroom

#reconstitution 2026-02-06

Friday27 messages6 participantstimes are UTC
Highlights from this day
  • vacuum_vic — if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration 17:11
  • lisbon_lot — give it 6 minutes at room temperature before you decide it has not dissolved 18:45
  • lisbon_lot — the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol 19:15
VV

how long after reconstituting before its actually dissolved

SS

if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way

CO

do not add more bac water to an already reconstituted vial, you have lost track of the concentration

not sterile water

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bac water

VV

if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration

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LL

update from 21 months ago: switched to a single concentration and have not miscalculated since

nope, redo it

CO

swirl dont shake

10mg vial
  + 1.0ml  -> 10.0 mg/ml   0.5mg =  5u   1mg = 10u
  + 2.0ml  ->  5.0 mg/ml   0.5mg = 10u   1mg = 20u
  + 2.5ml  ->  4.0 mg/ml   0.5mg = 12.5u (dont)
  + 5.0ml  ->  2.0 mg/ml   0.5mg = 25u   1mg = 50u
LL

give it 6 minutes at room temperature before you decide it has not dissolved

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LL

i swirl for about thirty seconds and then leave it. never had one fail to dissolve

sanity check my maths before i pin myself with something stupid

nobody here should be telling you a dose. we can check your arithmetic, thats a different thing, anyway

foam is bad

the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol

U-100 syringe: 1 unit = 0.01 ml
  10u = 0.10 ml
  25u = 0.25 ml
  50u = 0.50 ml
 100u = 1.00 ml
mg drawn = ml drawn x mg/ml
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VV

sorry to jump in 15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it

it foamed. is it ruined

VV

right so the meniscus thing is real. read it at eye level or you are guessing by a unit either way

TM

gross powder mass is not net peptide. water and counterion are in that number
10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use

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anyone else find the 2mg vials harder to get fully dissolved than the small ones