im getting bubbles in the barrel every single time, what am i doing wrong
#reconstitution 2026-01-04
Sunday24 messages6 participantstimes are UTC
Highlights from this day
- meniscus_mel — i keep a spare vial of bac water in the fridge purely so i never rush the maths 16:55
- triumph_watch — gross powder mass is not net peptide. water and counterion are in that number bacteriostatic water for anything multi-use. sterile water has no preservative in it at… 17:24
- meniscus_mel — if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way, thats just me 17:59
- triumph_watch — do not add more bac water to an already reconstituted vial, you have lost track of the concentration 10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a… 18:03
- point_two_five — if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration, happy to be corrected 20:07
you can absolutely just always use 2ml, and honestly most of us should
too dilute imo
how much do i lose to dead space with a 31G pin
ok so can i add more bac water later to a vial ive already reconstituted
thats 5mg/ml
[edited]i have never once regretted using a rounder number and a slightly bigger volume, i could be wrong
i keep a spare vial of bac water in the fridge purely so i never rush the maths
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swirl dont shake
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
gross powder mass is not net peptide. water and counterion are in that number
bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
is there any reason not to just always use 2ml
*Janoshik not the other one
quick one one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way, thats just me
bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
do not add more bac water to an already reconstituted vial, you have lost track of the concentration
10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100
lyophilised powder is far more robust than people think. it is the solution that is fragile
yep 10 units
one unit is 0.01ml
clear or bin it
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if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration, happy to be corrected
thats the vacuum
it should be completely clear with no floaters. cloudy means stop, not advice obviously