genuine question the jewellery scale is a good habit but calibrate it, they drift badly
#reconstitution 2025-12-01
Monday55 messages12 participantstimes are UTC
Highlights from this day
- VialBot — Digest for the week of 2025-07-12 has been published. 16:31
- fake_tracking — coming back after 5 months, has the pinned recon volume advice changed 17:08
- forty_units — quick one i keep a spare vial of bac water in the fridge purely so i never rush the maths 17:12
- lyophile_liv — sorry to jump in does temperature of the bac water matter at all 19:02
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
anyone else find the 5mg vials harder to get fully dissolved than the small ones
gross powder mass is not net peptide. water and counterion are in that number
the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol, ask me again in a month
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
Digest for the week of 2025-07-12 has been published.
✅7
label the date too. you will not remember which vial is which by week 12
*week 16 not week 8
read it at eye level
coming back to this give it 7 minutes at room temperature before you decide it has not dissolved
im getting bubbles in the barrel every single time, what am i doing wrong
🔥3
aim the diluent at the glass wall, not at the powder. it goes in gentler
[edited]🔥7
a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
coming back after 5 months, has the pinned recon volume advice changed
✅16📉7😂11
15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it
slightly off topic but dead space in a standard insulin syringe hub is small but it is not nothing over a month
my rule is one concentration for everything so i never have to think about it at 7am
quick one i keep a spare vial of bac water in the fridge purely so i never rush the maths
U-100 syringe: 1 unit = 0.01 ml
10u = 0.10 ml
25u = 0.25 ml
50u = 0.50 ml
100u = 1.00 ml
mg drawn = ml drawn x mg/mllabel the vial
while im here is it normal for the cake to look slightly collapsed on one side
30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
i write the mg/ml on the vial with a sharpie the moment i reconstitute it
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
😂4
nope, redo it
the meniscus thing is real. read it at eye level or you are guessing by a unit either way
i was wrong about this last year: it is fine to draw cold, it just stings more going in, i could be wrong
❤️7📉4
do i swirl for a specific length of time or just until its clear
do you let it come to room temp before drawing or draw it cold
😂1⚠️3
my vial pulled the water in on its own, is that the vacuum or did i do something
the powder is stuck to the stopper, do i care
does it matter if i inject the bac water straight at the powder
took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience
bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
update on the earlier thing how much do i lose to dead space with a 31G pin
10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100
clear or bin it
foam is bad
a slightly collapsed cake is cosmetic. a puddle in the bottom is not
🧊3👀1
thats the vacuum
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
[edited]write the date on
sorry to jump in does temperature of the bac water matter at all
📈17
swirl dont shake
if i add 3ml to a 40mg vial what concentration am i actually at
what do you do with the last 5 units when you cant draw them cleanly
*Janoshik not the other one
pick the volume so your dose is a whole number of units. thats the entire trick
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
how do you all measure a half unit, or do you just not
update from 20 months ago: switched to a single concentration and have not miscalculated since
thats fine
it foamed. is it ruined
the meniscus thing is real. read it at eye level or you are guessing by a unit either way
too dilute imo
🎉8📈1
i use bac water even for a single-use vial because it stings less, which is not the reason it exists
anyone got a photo of what a properly dissolved vial looks like
round numbers
🎉6🧪8