if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
#reconstitution 2025-10-29
Wednesday39 messages8 participantstimes are UTC
Highlights from this day
- lot_number_lou — can i add more bac water later to a vial ive already reconstituted 20:42
- VialBot — Verification log updated: CPC — evidence added, status unchanged. 20:46
- lot_number_lou — is there any reason not to just always use 2ml 21:08
- noct.titrate — my vial pulled the water in on its own, is that the vacuum or did i do something 21:22
- noct.titrate — swirl it. do not shake it. the foam is denatured protein and you are not getting it back 21:40
for the archive do you draw up with the same pin you inject with
the meniscus thing is real. read it at eye level or you are guessing by a unit either way
quick one how long after reconstituting before its actually dissolved
thats 5mg/ml
can i add more bac water later to a vial ive already reconstituted
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bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
Verification log updated: CPC — evidence added, status unchanged.
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it should be completely clear with no floaters. cloudy means stop
medutest-report-b-0114.pdf
3 pages · 188 KB · not retained in the public archive
weighing the vial before you reconstitute is the only way to catch an underfill
15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it
thats the vacuum
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is there any reason not to just always use 2ml
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slightly off topic but 30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
coming back to this what do you do with the last 5 units when you cant draw them cleanly
nobody here should be telling you a dose. we can check your arithmetic, thats a different thing, we shall see
coming back to this i swirl for about thirty seconds and then leave it. never had one fail to dissolve
my vial pulled the water in on its own, is that the vacuum or did i do something
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how do you all measure a half unit, or do you just not
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
check your maths
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swirl it. do not shake it. the foam is denatured protein and you are not getting it back
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gross powder mass is not net peptide. water and counterion are in that number
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
[edited]whats the smallest volume you would trust yourself to measure on a u100
if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space
sorry to jump in give it 2 minutes at room temperature before you decide it has not dissolved
yep 10 units
[edited]the last few units are a real loss. i accept it rather than tilting the vial and drawing air
if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
the powder is stuck to the stopper, do i care
sanity check my maths before i pin myself with something stupid
i keep a spare vial of bac water in the fridge purely so i never rush the maths
for the archive you can absolutely just always use 2ml, and honestly most of us should
pick the volume so your dose is a whole number of units. thats the entire trick
thats fine
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do not add more bac water to an already reconstituted vial, you have lost track of the concentration
aim the diluent at the glass wall, not at the powder. it goes in gentler, from memory
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took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience