ok so the powder is stuck to the stopper, do i care
#reconstitution 2025-10-18
Saturday49 messages12 participantstimes are UTC
Highlights from this day
- VialBot — Digest for the week of 2025-08-15 has been published. 20:35
- hiccup_hollis — the meniscus thing is real. read it at eye level or you are guessing by a unit either way the jewellery scale is a good habit but calibrate it, they drift badly 20:42
- hiccup_hollis — 10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use 20:46
- batch_bandit — i keep a spare vial of bac water in the fridge purely so i never rush the maths the jewellery scale is a good habit but calibrate it, they drift badly 21:14
- karl_fischer — update on the earlier thing do not add more bac water to an already reconstituted vial, you have lost track of the concentration 21:43
yep 10 units
gross powder mass is not net peptide. water and counterion are in that number
anyone else find the 30mg vials harder to get fully dissolved than the small ones
foam is bad
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
slightly off topic but you can absolutely just always use 2ml, and honestly most of us should
one unit is 0.01ml
does temperature of the bac water matter at all
read it at eye level
thats the vacuum
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
it should be completely clear with no floaters. cloudy means stop
Digest for the week of 2025-08-15 has been published.
thats 5mg/ml
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10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100, from memory
the meniscus thing is real. read it at eye level or you are guessing by a unit either way
the jewellery scale is a good habit but calibrate it, they drift badly
clear or bin it
10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
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round numbers
dead space
swirl dont shake
sorry to jump in how do you all measure a half unit, or do you just not
i keep a spare vial of bac water in the fridge purely so i never rush the maths
the jewellery scale is a good habit but calibrate it, they drift badly
do you let it come to room temp before drawing or draw it cold
[edited]weigh it first
does it matter if i inject the bac water straight at the powder
ok so my 10mg vial weighed 11.4g on the jewellery scale, is that wrong
if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
check your maths
update on the earlier thing do not add more bac water to an already reconstituted vial, you have lost track of the concentration
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my rule is one concentration for everything so i never have to think about it at 7am
i swirl for about thirty seconds and then leave it. never had one fail to dissolve, i could be wrong
took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience
do you draw up with the same pin you inject with
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
bac water
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
i write the mg/ml on the vial with a sharpie the moment i reconstitute it
anyone worked out a recon volume that puts 15mg on a round unit mark
pick the volume so your dose is a whole number of units. thats the entire trick
do i swirl for a specific length of time or just until its clear
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
weighing the vial before you reconstitute is the only way to catch an underfill
i was wrong about this last year: it is fine to draw cold, it just stings more going in
if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space