vialroom

#reconstitution 2025-08-05

Tuesday35 messages8 participantstimes are UTC
Highlights from this day
  • quiet.hours — does it matter if i inject the bac water straight at the powder 18:49
  • quiet.hours — swirl it. do not shake it. the foam is denatured protein and you are not getting it back 19:26
  • deadspace — aim the diluent at the glass wall, not at the powder. it goes in gentler 21:49
PN

if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right

yep 10 units

QH

gross powder mass is not net peptide. water and counterion are in that number

QH

does it matter if i inject the bac water straight at the powder

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PN

coming back to this a slightly collapsed cake is cosmetic. a puddle in the bottom is not

how long after reconstituting before its actually dissolved

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a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with

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19:13quiet.hours pinned a message to this channel
QH

you can absolutely just always use 2ml, and honestly most of us should

QH

swirl it. do not shake it. the foam is denatured protein and you are not getting it back

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anyone worked out a recon volume that puts 0.5mg on a round unit mark

TA

whats the smallest volume you would trust yourself to measure on a u100

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DD

unrelated but what do you do with the last 30 units when you cant draw them cleanly

*VendorInvestigate not the other one

DD

took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience

MM

pick the volume so your dose is a whole number of units. thats the entire trick

read it at eye level

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DD

for the archive 5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose

MM

one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that

MP

i write the mg/ml on the vial with a sharpie the moment i reconstitute it

too dilute imo

does temperature of the bac water matter at all

thats the vacuum

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DE

aim the diluent at the glass wall, not at the powder. it goes in gentler

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