#reconstitution 2025-07-13

Sunday16 messages6 participantstimes are UTC

Highlights from this day

chain_of_custody — you can absolutely just always use 2ml, and honestly most of us should, i have it written down somewhere 13:29
chain_of_custody — swirl it. do not shake it. the foam is denatured protein and you are not getting it back, still working it out 13:30
chain_of_custody — i have never once regretted using a rounder number and a slightly bigger volume 13:54
swirl_not_shake — a slightly collapsed cake is cosmetic. a puddle in the bottom is not 14:34
typosquat_tay — anyone else find the 5mg vials harder to get fully dissolved than the small ones 14:52

round numbers

you can absolutely just always use 2ml, and honestly most of us should, i have it written down somewhere

swirl it. do not shake it. the foam is denatured protein and you are not getting it back, still working it out

🎉1

check your maths

can i add more bac water later to a vial ive already reconstituted

i have never once regretted using a rounder number and a slightly bigger volume

✅2📉1

one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that

a slightly collapsed cake is cosmetic. a puddle in the bottom is not

U-100 syringe: 1 unit = 0.01 ml
  10u = 0.10 ml
  25u = 0.25 ml
  50u = 0.50 ml
 100u = 1.00 ml
mg drawn = ml drawn x mg/ml

do you let it come to room temp before drawing or draw it cold

one unit is 0.01ml

aim the diluent at the glass wall, not at the powder. it goes in gentler, still working it out

foam is bad

anyone else find the 5mg vials harder to get fully dissolved than the small ones

🔥3

took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience

20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy

my vial pulled the water in on its own, is that the vacuum or did i do something