how long after reconstituting before its actually dissolved
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#reconstitution 2025-06-05
Thursday46 messages12 participantstimes are UTC
Highlights from this day
- point_two_five — how long after reconstituting before its actually dissolved 19:30
- snac_and_water — a slightly collapsed cake is cosmetic. a puddle in the bottom is not 19:48
- VialBot — Testing queue: 6 submissions open, 34 awaiting dispatch. 19:56
- vial_ledger — genuine question if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space 20:50
- non_scale_win — dead space in a standard insulin syringe hub is small but it is not nothing over a month 21:10
does benzyl alcohol matter if im finishing the vial in one go
is it normal for the cake to look slightly collapsed on one side
nope, redo it
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follow up i keep a spare vial of bac water in the fridge purely so i never rush the maths
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a slightly collapsed cake is cosmetic. a puddle in the bottom is not
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whats the smallest volume you would trust yourself to measure on a u100
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Testing queue: 6 submissions open, 34 awaiting dispatch.
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can i add more bac water later to a vial ive already reconstituted
update from 21 months ago: switched to a single concentration and have not miscalculated since
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
read it at eye level
label the vial
thats fine
30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
do you draw up with the same pin you inject with
thats 5mg/ml
anyone worked out a recon volume that puts 7.5mg on a round unit mark
lyophilised powder is far more robust than people think. it is the solution that is fragile
genuine question if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space
coa-b-0114.pdf
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ok so do not add more bac water to an already reconstituted vial, you have lost track of the concentration
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
gross powder mass is not net peptide. water and counterion are in that number, i have it written down somewhere
im getting bubbles in the barrel every single time, what am i doing wrong
pick the volume so your dose is a whole number of units. thats the entire trick
label the date too. you will not remember which vial is which by week 12
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dead space in a standard insulin syringe hub is small but it is not nothing over a month
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update from 17 months ago: switched to a single concentration and have not miscalculated since
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
follow up the meniscus thing is real. read it at eye level or you are guessing by a unit either way, still working it out
*Janoshik not the other one
one unit is 0.01ml
what do you do with the last 30 units when you cant draw them cleanly
not sterile water
[edited]the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol
ok so i swirl for about thirty seconds and then leave it. never had one fail to dissolve
swirl dont shake
write the date on
my rule is one concentration for everything so i never have to think about it at 7am, anyway
temperature of the diluent does not matter. how fast you push it in does
is there any reason not to just always use 2ml
do you let it come to room temp before drawing or draw it cold
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
sorry to jump in why does everyone say 2ml specifically and not 1ml
how do you all measure a half unit, or do you just not
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way, thats one data point
does temperature of the bac water matter at all