aim the diluent at the glass wall, not at the powder. it goes in gentler
#reconstitution 2025-04-19
Saturday23 messages6 participantstimes are UTC
Highlights from this day
- bac_water_bill — do i swirl for a specific length of time or just until its clear do not add more bac water to an already reconstituted vial, you have lost track of the concentration 17:54
- spreadsheet_stu — swirl it. do not shake it. the foam is denatured protein and you are not getting it back 18:05
- stack_sceptic — whats the smallest volume you would trust yourself to measure on a u100 19:58
one unit is 0.01ml
unrelated but do you let it come to room temp before drawing or draw it cold
if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space
do i swirl for a specific length of time or just until its clear
do not add more bac water to an already reconstituted vial, you have lost track of the concentration
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nope, redo it
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
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if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
quick one if i add 2ml to a 20mg vial what concentration am i actually at
anyone got a photo of what a properly dissolved vial looks like
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
unrelated but how long after reconstituting before its actually dissolved
update from 3 months ago: switched to a single concentration and have not miscalculated since
it should be completely clear with no floaters. cloudy means stop
[edited]quick one is a slightly cloudy solution ever ok or is that always a bin it
i keep a spare vial of bac water in the fridge purely so i never rush the maths
how much do i lose to dead space with a 30G pin
do not add more bac water to an already reconstituted vial, you have lost track of the concentration
check your maths
for the archive 20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
what do you do with the last 40 units when you cant draw them cleanly
whats the smallest volume you would trust yourself to measure on a u100
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my 10mg vial weighed 11.4g on the jewellery scale, is that wrong
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically, still working it out
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose