read it at eye level
#reconstitution 2025-01-12
Sunday41 messages11 participantstimes are UTC
Highlights from this day
- swab_dry_first — do not add more bac water to an already reconstituted vial, you have lost track of the concentration 13:05
- spreadsheet_stu — i have never once regretted using a rounder number and a slightly bigger volume 17:00
- pin29g — if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way, for what its worth 17:50
- spreadsheet_stu — sanity check my maths before i pin myself with something stupid 17:52
it should be completely clear with no floaters. cloudy means stop
10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
weighing the vial before you reconstitute is the only way to catch an underfill
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round numbers
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
if i add 3ml to a 15mg vial what concentration am i actually at
bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
write the date on
the jewellery scale is a good habit but calibrate it, they drift badly
do not add more bac water to an already reconstituted vial, you have lost track of the concentration
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one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically, we shall see
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genuine question 15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it
aim the diluent at the glass wall, not at the powder. it goes in gentler
swirl dont shake
bac water
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weigh it first
is it normal for the cake to look slightly collapsed on one side
the meniscus thing is real. read it at eye level or you are guessing by a unit either way
nobody here should be telling you a dose. we can check your arithmetic, thats a different thing
a slightly collapsed cake is cosmetic. a puddle in the bottom is not
Reminder set. I will post here in 5 days.
New independent result logged — HJ, lot D-0718, purity 98.6% (PeptideMeter).
30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right, we shall see
janoshik-kp-0925.pdf
2 pages · 804 KB · not retained in the public archive
gross powder mass is not net peptide. water and counterion are in that number
dead space in a standard insulin syringe hub is small but it is not nothing over a month
i keep a spare vial of bac water in the fridge purely so i never rush the maths
thats fine
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karl_fischerbenzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically, we shall see
i have never once regretted using a rounder number and a slightly bigger volume
10mg vial
+ 1.0ml -> 10.0 mg/ml 0.5mg = 5u 1mg = 10u
+ 2.0ml -> 5.0 mg/ml 0.5mg = 10u 1mg = 20u
+ 2.5ml -> 4.0 mg/ml 0.5mg = 12.5u (dont)
+ 5.0ml -> 2.0 mg/ml 0.5mg = 25u 1mg = 50u📉3😂1🤝1
update on the earlier thing a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
does it matter if i inject the bac water straight at the powder
my rule is one concentration for everything so i never have to think about it at 7am, for what its worth
too dilute imo
swab_dry_firstdo not add more bac water to an already reconstituted vial, you have lost track of the…
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way, for what its worth
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sanity check my maths before i pin myself with something stupid
janoshik-a-2601.pdf
2 pages · 554 KB · not retained in the public archive
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give it 4 minutes at room temperature before you decide it has not dissolved
foam is bad
do i swirl for a specific length of time or just until its clear