the powder is stuck to the stopper, do i care
#reconstitution 2024-04-13
Saturday31 messages6 participantstimes are UTC
Highlights from this day
- mg_per_ml — gross powder mass is not net peptide. water and counterion are in that number 20:04
- still_here_2024 — my rule is one concentration for everything so i never have to think about it at 7am 21:14
- retention_time — i keep a spare vial of bac water in the fridge purely so i never rush the maths 22:04
whats the smallest volume you would trust yourself to measure on a u100
you can absolutely just always use 2ml, and honestly most of us should
clear or bin it
anyone worked out a recon volume that puts 2.4mg on a round unit mark
sanity check my maths before i pin myself with something stupid
a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
thats 5mg/ml
right so the last few units are a real loss. i accept it rather than tilting the vial and drawing air, we shall see
right so 20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
gross powder mass is not net peptide. water and counterion are in that number
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weigh it first
lyophilised powder is far more robust than people think. it is the solution that is fragile
is a slightly cloudy solution ever ok or is that always a bin it
the jewellery scale is a good habit but calibrate it, they drift badly
thats the vacuum
anyone got a photo of what a properly dissolved vial looks like
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does benzyl alcohol matter if im finishing the vial in one go
ok so temperature of the diluent does not matter. how fast you push it in does
i was wrong about this last year: it is fine to draw cold, it just stings more going in
thats fine
my rule is one concentration for everything so i never have to think about it at 7am
10mg vial
+ 1.0ml -> 10.0 mg/ml 0.5mg = 5u 1mg = 10u
+ 2.0ml -> 5.0 mg/ml 0.5mg = 10u 1mg = 20u
+ 2.5ml -> 4.0 mg/ml 0.5mg = 12.5u (dont)
+ 5.0ml -> 2.0 mg/ml 0.5mg = 25u 1mg = 50uif you cannot read the mark confidently, your volume is too small. redo it at a lower concentration, take that with a pinch of salt
10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
does temperature of the bac water matter at all
anyone else find the 30mg vials harder to get fully dissolved than the small ones
a slightly collapsed cake is cosmetic. a puddle in the bottom is not
i keep a spare vial of bac water in the fridge purely so i never rush the maths
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unrelated but 30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
it should be completely clear with no floaters. cloudy means stop