a certificate with a number and no chromatogram is asking you to trust the integration you cannot see
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Spun off from a message in #hplc-massspec on 2026-05-30. 11 messages, 3 participants.
a certificate with a number and no chromatogram is asking you to trust the integration you cannot see
thats the solvent peak
why do two labs integrate the same peak differently
retention time is a hint
214nm sees the amide backbone so it sees everything. 280 only sees aromatics
ms/ms or it isnt identity
nobody runs enough blanks, including me
shallow gradient
if the lab reports area percent without stating the wavelength, the number is less useful than it looks
if somebody says they ran a mass spec and does not show you a spectrum, that is a claim, not a result
214nm sees the amide backbone so it sees everything. 280 only sees aromatics