whats a normal gradient for a peptide this size
Thread · #hplc-massspec · 2025-12-03
baseline is drifting badly, is that the column or
Spun off from a message in #hplc-massspec on 2025-12-03. 7 messages, 4 participants.
The message this came from
- drawup_dee — baseline is drifting badly, is that the column or the mobile phase 22:55
ms/ms or it isnt identity
truncations show as a mass deficit matching one or more residues. you need the sequence to say which
214nm sees the amide backbone so it sees everything. 280 only sees aromatics
⚠️11🤝14
nobody runs enough blanks, including me
if the lab reports area percent without stating the wavelength, the number is less useful than it looks
whats a plus one dalton shift usually