co-elution is the failure mode that a beautiful single peak is most likely to be hiding
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#hplc-massspec 2026-05-11
Monday15 messages5 participantstimes are UTC
Highlights from this day
- ms_ms_mira — co-elution is the failure mode that a beautiful single peak is most likely to be hiding 06:00
- ms_ms_mira — the honest position is that hobby-level interpretation of a chromatogram is worth something but not much 06:14
- ms_ms_mira — while im here update from 2 months ago: switched to asking for the chromatogram rather than the number and it changed everything 06:22
- VialBot — Reminder for HPLC_Hank: dose day is today. Set 7 days ago. 07:22
- HPLC_Hank — is a tailing factor of anything specific a problem i have had a lot where the mass was exactly right and the purity was 96.8. wrong question, right answer 07:24
deamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant
do you trust an area percent from a shallow gradient
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coming back after 18 months, what changed in how you read these
the honest position is that hobby-level interpretation of a chromatogram is worth something but not much
while im here update from 2 months ago: switched to asking for the chromatogram rather than the number and it changed everything
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does retention time on its own tell you what the compound is
the first peak is the solvent. it is always the solvent
i have a number at about a third of what i expected, is that a charge state
baseline drift on a gradient is normal. baseline drift on an isocratic hold is your column
a shoulder on a peak is information, and usually bad information, from memory
how do you tell co-elution from a clean single peak
integration difference
Reminder for HPLC_Hank: dose day is today. Set 7 days ago.
is a tailing factor of anything specific a problem
i have had a lot where the mass was exactly right and the purity was 96.8. wrong question, right answer