thats co-elution
#hplc-massspec 2026-02-26
Thursday17 messages3 participantstimes are UTC
Highlights from this day
- LC_MS_Lena — right so how do you spot a deletion in a sequence from mass alone 21:38
- batch_bandit — the first peak is the solvent. it is always the solvent 22:07
- batch_bandit — a dimer usually shows up late and at roughly double the mass. the chromatogram alone will not tell you, still working it out 22:49
right so how do you spot a deletion in a sequence from mass alone
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nobody runs enough blanks, including me
run a blank
is there any point running a blank if the sample is the question
coming back after 19 months, what changed in how you read these
whats a plus one dalton shift usually
the first peak is the solvent. it is always the solvent
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integration choices move the number by a few tenths easily. that is most of your inter-lab variance
ms/ms or it isnt identity
thats a charge state
*that should say weekly
show me the spectrum
whats the void volume peak, is that always solvent
do the arithmetic
a dimer usually shows up late and at roughly double the mass. the chromatogram alone will not tell you, still working it out
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has anyone ever had a result where the mass was right and the purity was wrong