run a blank
#hplc-massspec 2026-01-04
Sunday13 messages3 participantstimes are UTC
Highlights from this day
- rotate_the_site — for the archive tirzepatide is about 4813.5 Da, so 2+ is near 2407.8 and 3+ near 1605.5 co-elution is the failure mode that a beautiful single peak is most likely to… 23:35
- third_shift — is there any point running a blank if the sample is the question 23:43
- vacuum_vic — a warm transit can put a visible degradant peak on a chromatogram. that is what 8 days at ambient does 23:44
plus 0.98 is deamidation
baseline drift on a gradient is normal. baseline drift on an isocratic hold is your column
thats the solvent peak
two labs a point or two apart on the same lot is normal and gets mistaken for one of them being wrong constantly
does retention time on its own tell you what the compound is
for the archive tirzepatide is about 4813.5 Da, so 2+ is near 2407.8 and 3+ near 1605.5
co-elution is the failure mode that a beautiful single peak is most likely to be hiding
has anyone ever had a result where the mass was right and the purity was wrong
show me the spectrum
is there any point running a blank if the sample is the question
✅10
a warm transit can put a visible degradant peak on a chromatogram. that is what 8 days at ambient does
🔥2🤝7👀3
ms/ms sequencing is the only thing i would call identity. everything else is circumstantial
plus 16 is oxidation