thats a charge state
#hplc-massspec 2025-12-12
Friday15 messages5 participantstimes are UTC
Highlights from this day
- VialBot — Batch lookup A-2418: 5 independent reports on file, earliest 2025-09-28. 07:51
- zoya_zeros — if somebody says they ran a mass spec and does not show you a spectrum, that is a claim, not a result 08:36
- zoya_zeros — a shoulder on a peak is information, and usually bad information update from 18 months ago: switched to asking for the chromatogram rather than the number and it… 08:40
plus 0.98 is deamidation
thats the solvent peak
👀1
ms/ms or it isnt identity
⚠️1
i was wrong about tailing factor. it is usually the injection or the pH, not the column
a dimer usually shows up late and at roughly double the mass. the chromatogram alone will not tell you
Batch lookup A-2418: 5 independent reports on file, earliest 2025-09-28.
🎉1👀1
shallow gradients hide related substances behind the main peak. a steeper one separates and looks worse, honestly
coming back to this 214nm sees the amide backbone so it sees everything. 280 only sees aromatics
within inter-lab range
if somebody says they ran a mass spec and does not show you a spectrum, that is a claim, not a result
fridge-thermometer.png
800 × 800 · 914 KB · not retained in the public archive
a certificate with a number and no chromatogram is asking you to trust the integration you cannot see
how do you tell co-elution from a clean single peak
a certificate with a number and no chromatogram is asking you to trust the integration you cannot see
a shoulder on a peak is information, and usually bad information
update from 18 months ago: switched to asking for the chromatogram rather than the number and it changed everything
is a tailing factor of anything specific a problem