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#hplc-massspec 2025-11-07

Friday11 messages3 participantstimes are UTC
Highlights from this day
  • zoya_zeros — is a tailing factor of anything specific a problem two labs a point or two apart on the same lot is normal and gets mistaken for one of them being wrong constantly 23:33
  • triple_ag_tom — the first peak is the solvent. it is always the solvent 23:37
  • triple_ag_tom — monoisotopic and average diverge more the bigger the molecule. say which one you are quoting 23:41
  • triple_ag_tom — is monoisotopic or average mass the one on most certificates 23:42
  • truncation_tru — retention time is a hint, not a fingerprint. two different peptides can co-elute perfectly happily, for what its worth 23:51

plus 0.98 is deamidation

show me the spectrum

is a tailing factor of anything specific a problem
two labs a point or two apart on the same lot is normal and gets mistaken for one of them being wrong constantly

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whats the void volume peak, is that always solvent

monoisotopic and average diverge more the bigger the molecule. say which one you are quoting

within inter-lab range

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TT

retention time is a hint, not a fingerprint. two different peptides can co-elute perfectly happily, for what its worth

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why does integration change the number so much