thats a charge state
#hplc-massspec 2025-06-16
Monday11 messages4 participantstimes are UTC
Highlights from this day
- eight_mm — is monoisotopic or average mass the one on most certificates 14:24
- salt_bridge — a certificate with a number and no chromatogram is asking you to trust the integration you cannot see 14:26
- salt_bridge — the first peak is the solvent. it is always the solvent, anyway 14:32
- HPLC_Hank — how do you spot a deletion in a sequence from mass alone retention time is a hint, not a fingerprint. two different peptides can co-elute perfectly happily 15:17
- panel_before_after — truncations show as a mass deficit matching one or more residues. you need the sequence to say which 15:57
is monoisotopic or average mass the one on most certificates
[edited]a certificate with a number and no chromatogram is asking you to trust the integration you cannot see
within inter-lab range
shallow gradient
the first peak is the solvent. it is always the solvent, anyway
retention time is a hint
plus 0.98 is deamidation
how do you spot a deletion in a sequence from mass alone
retention time is a hint, not a fingerprint. two different peptides can co-elute perfectly happily
M = 4113.6
[M+2H]2+ = (4113.6 + 2.02) / 2 = 2057.8
[M+3H]3+ = (4113.6 + 3.02) / 3 = 1372.2
observed 1372.3 -> consistentnobody runs enough blanks, including me
truncations show as a mass deficit matching one or more residues. you need the sequence to say which