#hplc-massspec 2025-04-14

update on the earlier thing 214nm sees the amide backbone so it sees everything. 280 only sees aromatics

i have a number at about a third of what i expected, is that a charge state

can you tell a dimer from the chromatogram alone

the first peak is the solvent. it is always the solvent

if the lab reports area percent without stating the wavelength, the number is less useful than it looks

deamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant

retention time is a hint

plus 0.98 is deamidation

Purity check: no report on file for lot A-2418. Nothing logged either way.

i asked Medutest for the raw trace and they sent it. that is the right answer to that question, thats just me

the honest position is that hobby-level interpretation of a chromatogram is worth something but not much