the honest position is that hobby-level interpretation of a chromatogram is worth something but not much
#hplc-massspec 2025-01-24
Friday11 messages4 participantstimes are UTC
Highlights from this day
- VialBot — Purity check: no report on file for lot E-2205. Nothing logged either way. 08:00
- HPLC_Hank — plus sixteen, is that always oxidation 08:33
- low_and_slow — you need more sample for identity than for purity. plan for that when you post it, i could be wrong 08:35
- low_and_slow — slightly off topic but i asked Medutest for the raw trace and they sent it. that is the right answer to that question 09:35
ms/ms sequencing is the only thing i would call identity. everything else is circumstantial
i have a number at about a third of what i expected, is that a charge state
*that should say weekly
shallow gradient
Purity check: no report on file for lot E-2205. Nothing logged either way.
plus sixteen, is that always oxidation
📈6
you need more sample for identity than for purity. plan for that when you post it, i could be wrong
👍5💀11📉7
deamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant
baseline drift on a gradient is normal. baseline drift on an isocratic hold is your column
slightly off topic but i asked Medutest for the raw trace and they sent it. that is the right answer to that question