if somebody says they ran a mass spec and does not show you a spectrum, that is a claim, not a result
#hplc-massspec 2024-11-05
Tuesday8 messages2 participantstimes are UTC
Highlights from this day
- cagri_curious — if somebody says they ran a mass spec and does not show you a spectrum, that is a claim, not a result 11:59
- cagri_curious — update on the earlier thing a warm transit can put a visible degradant peak on a chromatogram. that is what 13 days at ambient does 12:59
- cagri_curious — how much sample does a lab actually need for identity as well as purity 13:00
- third_shift — you need more sample for identity than for purity. plan for that when you post it 14:02
- third_shift — sorry to jump in retention time is a hint, not a fingerprint. two different peptides can co-elute perfectly happily 14:45
update on the earlier thing a warm transit can put a visible degradant peak on a chromatogram. that is what 13 days at ambient does
how much sample does a lab actually need for identity as well as purity
nobody runs enough blanks, including me
you need more sample for identity than for purity. plan for that when you post it
baseline is drifting badly, is that the column or the mobile phase
thats co-elution
sorry to jump in retention time is a hint, not a fingerprint. two different peptides can co-elute perfectly happily